Figure 5.

Ex vivo culture of monocytes leads to an altered phenotype. (A) Representative flow cytometry plots showing proportions of monocyte subsets from healthy controls (B) following incubation in culture for 24 h and (C) following incubation in culture in the presence of LPS for 24 h. (D) 24 h culture with LPS results in a smaller proportion of CD14⁺⁺CD16⁺ monocytes (p = 0.003) but this population is restored with prolonged incubation in culture (ns = not statistically significant). (E) Addition of TMI005 (1 μg/mL) had no effect on LPS-mediated inhibition of monocyte maturation ex vivo, however (F) addition of LPS to PBMCs following 24 h incubation in unstimulated medium led to a reduction of intermediate monocytes in an ADAM17-dependent manner.