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. 2019 Aug 27;10:2030. doi: 10.3389/fimmu.2019.02030

Figure 6.

Figure 6

IL-18 contributes to NK cell activation and IFNγ secretion in a B. pertussis-stimulated mo-MΦ/NK co-culture. (A,B) Mo-MΦ/NK co-cultures were stimulated with B. pertussis (B4393, MOI = 10, dashed bars) for 22 h in the presence of a caspase inhibitor (squares). (A) IFNγ was measured in the supernatant and (B) IL-2Rα expression was determined on CD56+CD3− NK cells using flow cytometry. Data is shown as relative to the cultures stimulated with B. pertussis in the absence of the caspase inhibitor (dots). (C,D) Mo-MΦ/NK co-cultures were stimulated with B. pertussis (B4393, MOI = 10, dashed bars) for 22 h in the presence of IL-18 blocking antibodies (triangles) or isotype control hIgA2 (dots). (C) IFNγ was measured in the supernatant and (D) IL-2Rα expression was determined on CD56+CD3− NK cells using flow cytometry. Data is shown as relative to the cultures stimulated with B. pertussis in the presence of hIgA2 (n = 4). Results are expressed as medians with interquartile range. Black dots, squares and triangles represent values of individual donors.