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. 2019 Jul 28;18(5):e13016. doi: 10.1111/acel.13016

Figure 3.

Figure 3

β‐Lap restores the NAD+ and SIRT1 activity in cochlear tissues. (a) NAD+ was extracted from cochlear tissues, and changes in NAD+ levels were measured using a fluorescent NAD+ detection kit. (b) PARP activity was assayed using the PARP assay kit. (c) Intracellular ATP levels in the cochlear tissue were measured using ATP assay kit, on a luminometer. (d) SIRT1 activity was measured using SIRT1 assay kit. (e) Level of SIRT1 protein was analyzed by Western blotting. (f) Level of SIRT1 mRNA was measured by qRT–PCR. (g) Level of miR‐34a was measured by qRT–PCR. (h) Levels of miR‐181, miR‐9, and miR‐146 were analyzed by qRT–PCR. Each value represents the mean ± SD (n = 5). *p < .05 versus 24‐month‐old ad libitum‐fed mice (AL group)