Figure 3.

Unlocked interferon signature response is IRF1-dependent. (A) qRT-PCR analysis of mRNA expression of the indicated interferon response genes in WT and Ifit1^−/− BMDM stimulated with TLR7 agonist R848 for 6 h in the presence or absence of MEK1/2 inhibitor (MEKi-U). Data are means ± SD from 3 to 5 independent experiments. (B) qRT-PCR analysis of Ifil1, Ifit2, Isgl5, and Batj2 mRNA expression in BMDM stimulated with RNA40 or RNA41 for 6 h in the presence or absence of MEKi-U. Data are means ± SD from 4 independent experiments. (C–E) Microarray analysis of mRNA expression in WT and Ifit1^−/− BMDM stimulated with TLR7 agonist R848 for 6 h in the presence or absence of MEKi-U. Heat map of IRF1-independent (C) or dependent (D) genes clustered using 1.5-fold difference cutoff. Gene set enrichment analysis of differentially expressed genes (E) are from all replicates. Quantified data are means ± SD from all experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 by unpaired Welch's t-test. n.s., not significant.