Figure 4.

IRF1 is unlocked through the activation of NF-κB pathway. (A–C) Immunoblot analysis and quantitative densitometry of the indicated proteins in lysates from BMDM activated with R848 in the presence or absence of MEKi-U, p38 MAPK inhibitor p38i, or JNK MAPK inhibitor JNKi (A), or MEKi-U, ERK inhibitor (ERKi), RSK inhibitor (RSKi), MNK inhibitor (MNKi), or MSK inhibitor (MSKi) (B), or MEKi-U, and NF-κB inhibitor NF-κBi (C) for 12 h (A,C) or 8 h (B). Blots are representative of 4 independent experiments. Molecular weight (kDa) markers are indicated on the right side of the blots. Quantified data are means ± SD from all experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 by unpaired Welch's t-test. n.s., not significant.