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. 2019 Jul 6;8(11):5232–5241. doi: 10.1002/cam4.2386

Figure 2.

Figure 2

Loss of Tyk2/TYK2 in JW23.3 murine MPNST and MPNST 724 cells leads to increased cell death. shTyk2‐infected JW23.3 cell lines and control shLacZ: (A) Western blot densitometry analysis measuring TYK2 protein. (B) Relative Expression of Tyk2 mRNA compared to Gapdh. (C) An Incucyte cell proliferation assay measuring confluence over time. (D) An Incucyte death assay measuring TOTOTM‐3 iodide fluorescence as an indicator of death over time. TYK2 knockout MPNST 724 cells and scramble control: (E) Western blot densitometry analysis of TYK2 protein. (F) Relative Expression of TYK2 mRNA compared to GAPDH. (G) An Incucyte cell proliferation assay measuring confluence over time. (H) An Incucyte death assay measuring TOTOTM‐3 iodide fluorescence as an indicator of death over time. (*P < 0.05, **P < 0.01, ***P < 0.001)