Figure 3.

Knockdown of Tyk2/TYK2 in JW23.3 murine MPNST and MPNST 724 cells affects expression of downstream targets. Western blot densitometry analysis on downstream targets of Tyk2/TYK2. Murine JW23.3 cell lines are depicted in blue/green, human MPNST 724 cell line are depicted in black/orange. (A and B) levels of TYK2 normalized to GAPDH (C and D) levels of p‐STAT1 normalized to total STAT1. (E and F) levels of p‐STAT3 normalized to total STAT3. (G and H) levels of Bcl‐2 normalized to GAPDH (I and J) levels of Cleaved Caspase‐3 normalized to Caspase 3 levels. (*P < 0.05, **P < 0.01, ***P < 0.001) All experiments were done in triplicate. Representative blots are shown