Figure 5.

ELISA: Expression of CXCR7 regulates VEGF secretion Notes: A, The levels of VEGF were significantly increased in SW480 and Caco‐2 cells overexpressing CXCR7. Treatment with inhibitors LY294002 and U0126 reduced the levels of VEGF. B, The levels of VEGF in CXCR7‐silencing SW480 and Caco‐2 cells were significantly reduced. ^* P < .05 and ^** P < .01 vs the respective NC group. ^# P < .05 vs the respective overexpression group. Data from each group are presented as the mean ± standard deviation of three independent experiments. Abbreviations: ELISA, enzyme‐linked immunosorbent assay; VEGF, vascular endothelial growth factor; NC, negative control; ns, no statistical significance; CXCR7, chemokine receptor 7; CXCR7OE, overexpression of CXCR7