Figure 5.

JQ1 treatment reverts the expression of some extracellular matrix (ECM) genes in aged muscle. (a) Snapshots of H3K27ac binding profiles on five selected ECM enhancers in 2‐, 10‐, and 20‐month‐old muscles. (b) Binding of H3K27ac and Brd4 (c) on selected ECM enhancers in the above muscles was measured by ChIP‐PCR. (d) RNA expression levels of the above selected ECM genes were quantified by RT‐qPCR. (e) Schematic overview of intraperitoneal injection of JQ1 and experimental design. (f) Down‐regulation of ECM genes (Col1a1, Timp2) in skeletal muscle treated with JQ1. (g) Brd4 or H3K27ac (h) binding on the above two genes was measured by ChIP‐PCR. The p‐value was determined by Student's t test: *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant