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. 2019 Sep 2;9:12645. doi: 10.1038/s41598-019-49164-3

Figure 1.

Figure 1

Flow cytometry analysis of immunoinfiltrates in colons. Epithelial (EP) and LP leukocyte population were isolated from colon samples as described in Materials and Methods section. (A) Myeloid population was investigated using panel 1 and cells were immunophenotyped as follow: macrophages as CD11b+/F4/80+; moncytes as CD11b+/F4/80−/Ly6C+; granulocytes as CD11b+/F4/80−/Ly6G+, dendritic cells as F4/80int/CD11cHi. (B) Lymphoid population was characterized using panel 2 and immunophenotyped as follow: T lymphocytes as CD11b−/CD3+/CD19−; B lymphocytes as CD11b−/CD3−/CD19+. (C) T lymphocytes were immunophenotyped on the basis of CD4+ or CD8+ gates. Bar graphs show mean values ± s.d. from at least three mice individually tested per group.