Fig. 1.

Non-colinear polyketide assembly lines for aureothin and neoaureothin biosynthesis. AurA and NorA catalyze two chain elongations. PNBA: p-nitrobenzoic acid, mM-CoA: methylmalonyl-CoA, M-CoA: malonyl-CoA, KS: ketosynthase, AT: acyltransferase, DH: dehydratase, ER: enoylreductase, KR: ketoreductase, ACP: acyl carrier protein, TE: thioesterase. The inactive domains in (AurA/NorA) and (AurC/NorC) are indicated with brackets. Color code in module indicates homologous proteins (except for AurB (cyan) and NorB (purple), for which different colors are used to distinguish the engineered site in Figs. 3d and 5.) These color codes correspond with those shown in Fig. 2c