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. 2019 Aug 27;9:809. doi: 10.3389/fonc.2019.00809

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Copyright © 2019 Feng, Wang, Liu, Li, Zhang, Zhuang, Yao, Liu, Li, Cao, Li, Gong, Li, Zhang and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Notch2 knockdown inhibited proliferation and migration of GH3 and primary GHoma cells. (A) Expression of Notch pathways after Notch2 knockdown in GH3 cells. Notch2, Cyclin D1, and DLL3 expression was reduced to 31.3, 54.7, and 37.4% of those in the scramble group after Notch2 knockdown. (B) The Effect of Notch2 shRNA on cell migration. The average number of migrated GH3 cells in knockdown group was 27.3 ± 8.4% compared to that in the scramble group by transwells experiment, and the migrated primary tumor cells was 38.9 ± 13.1%compared to that in the scramble group. (C) The Effect of Notch2 shRNA on cell viability. The reduction of cell viability of GH3 was time dependence. (D) Expression of genes associated with migration, invasion, and growth hormone synthesis. The mRNA of CORT in knockdown group was 0.032-folds of that in scramble group, and GHRH 0.04-folds, Snail 0.13-folds, Vimentin 0.042-folds, VEGF 0.172-folds, SSTR1 0.005-folds, SSTR2 0.0245-folds, and SSTR5 0.006-folds. Assays were performed in triplicate. P-values indicated by * and ** (< 0.05, < 0.01), ^# (< 0.05).