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. 2019 Aug 27;9:809. doi: 10.3389/fonc.2019.00809

Figure 4.

Figure 4

DAPT inhibited cell proliferation, growth hormone secretion, and invasion in vitro. (A) DAPT reduced the viability of GH3 cells and primary GHoma cells. The cell viability was reduced to 83.4 ± 7.6, 63.5 ± 5.3, and 59.4 ± 4.7% after 24, 48, and 72 h of 100 nM DAPT in GH3 cells, and 78 ± 7.1, 67 ± 6.3, 53 ± 4.7% in primary GHoma cell. (B) DAPT decreased growth hormone secretion in GH3 cell and primary GHoma cells. The levels of Growth hormone in GH3 cell culture were reduced to 84.3 ± 15 (20 nM) and 68.4 ± 14.5 (100 nM) mIU/ml after 24 h treatment, and 94.6 ± 18 (20 nM) and 58.9 ± 13 (100 nM) mIU/ml after 48 h treatment. The levels of Growth hormone in primary GHoma were 48.7 ± 5.3 (20 nM) and 48.9 ± 7.3 (100 nM) mIU/m after 24 h treatment, and 37.5 ± 4.2 (20 nM) and 34.7 ± 6.5 (100 nM) mIU/m after 48 h treatment. (C) DAPT inhibited the migration of GH3 cells and primary GHoma cells. (D) The average positive cells were reduced to 142 ± 35 and 94 ± 27 from 323 ± 64 after DAPT treatment in GH3 cell line, and 84 ± 23 and 42 ± 19 from 167 ± 35 in primary GHoma cells. Blue: positive cells. Assays were performed in triplicate. P-values indicated by * and ** (<0.05, <0.01).