Figure 2.

Amylin content in the hippocampi of hIAPP^−/− and hIAPP^+/– mice increased under a high-fat diet. (A) Representative images of amylin-positive particles in the DG of the hippocampus. White arrows indicate positive staining with the amylin antibody, and blue staining indicates the nucleus. (B) Six sections per mouse were stained, and positive particles were analyzed by using ImageJ software. L^−/− denotes hIAPP^−/− mice on a low-fat diet; L^+/– denotes hIAPP^+/– mice on a low-fat diet; H^−/− denotes hIAPP^−/− mice on a high-fat diet; H^+/– denotes hIAPP^+/– mice on a high-fat diet. n = 6, *p < 0.05, **p < 0.01, ***p < 0.001 compared with the L^−/− group.