Figure 5.

Aβ42 deposition in the hippocampi and prefrontal cortexes of hIAPP^−/− and hIAPP^−/+ mice were enhanced by a high-fat diet. (A) Representative images of Aβ42-positive staining in the hippocampal CA1 regions of hIAPP^−/− and hIAPP^+/– mice treated with a high/low-fat diet for 6 or 12 months. Black arrows indicate Aβ42-positive particles. (B) Six sections per mouse were stained, and semiquantitative analysis was performed using ImageJ software. (C) Representative images of Aβ42-positive staining in the prefrontal cortexes of hIAPP^−/− and hIAPP^+/– mice treated with a high/low-fat diet for 6 or 12 months. Black arrows indicate Aβ42-positive particles. (D) Six sections per mouse were stained, and semiquantitative analysis was performed using imageJ software. L^−/− denotes hIAPP^−/− mice on a low-fat diet; L^+/– denotes hIAPP^+/– mice on a low-fat diet; H^−/− denotes hIAPP^−/− mice on a high-fat diet; H^+/– denotes hIAPP^+/– mice on a high-fat diet. n = 6, *p < 0.05, **p < 0.01, ***p < 0.001 compared with the L^−/− group.