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. 2019 Oct;179:104991. doi: 10.1016/j.cmpb.2019.104991

Fig. A.2.

Fig A2

Detected ROIs for Dataset 1 using various segmentation strategies. The bar represents a width of 400 µm. ROIs, manual (a), ROIs, CALIMA (b), ROIs, Active Contours (FSNNAP) (c), ROIs, Otsu Thresholding + brightness-based declumping (CellProfiler) (d), ROIs, Thresholding + Watershedding (ImageJ) (e), ROIs, spatiotemporal Independent component analysis (SIMA) (f). The ROIs found manually are depicted in blue, the true positives in green, and the false positives in orange. The ROIs containing multiple cells are shown in purple and the false negatives in red. Note that an exception is made for the stICA method, as it allows regions to overlap. To avoid confusion, the segmentation outlines found by this method are shown as well.