Figure 1.

Heterogeneous Responses to Cisplatin in TNBC Cell Lines

(A) Indicated TNBC cell lines were treated with cisplatin for 72 h. Methyl thiazol tetrazolium (MTT) conversion was measured, and growth rate-adjusted drug responses (GR metrics) were plotted. Error bars indicate SEM of at least three independent experiments with three technical replicates each. MDA-MB-231 and MDA-MB-157 are called MB-231 and MB-157, respectively.

(B) Indicated TNBC cell lines were irradiated (10 Gy) or left untreated and analyzed for RAD51 foci 3 h later. Scale bar represents 10 μM.

(C) Indicated TNBC cell lines were treated with mitomycin C (MMC, 50 ng/mL) for 24 h. FANCD2 ubiquitination was assessed by western blotting.

(D) Characteristics of all 9 tested TNBC cell lines are listed. GR50 values for cisplatin were calculated from averages of three independent experiments. TP53, BRCA1, and BRCA2 mutation status was obtained from the Cosmic database.

See also Figure S1.