Figure 4.

The Absence of UCP2 in Colon Tumors Promotes Glutathione Oxidation and Protein Carbonylation and Depletes NADPH Levels

(A) Western blot analysis for 4-hydroxy-2-nonenal (4-HNE)-modified proteins in colon tumor (T) samples. Data indicate means ± SEMs (n = 5).

(B) Representative immunoblot of protein oxidation levels (total carbonyl groups with dinitrophenyl [DNP]) in colon T samples. Data indicate means ± SEMs (n = 6).

(C–E) Total glutathione (C), oxidized glutathione (GSSG) (D), and ratio of reduced glutathione (GSH) versus GSSG (E). Total GSH and GSSG were normalized by protein content. Data indicate means ± SEMs (Ucp2^+/+ T n = 7, Ucp2^−/− T n = 9).

(F–H) NADPH (F) and NADP⁺ (G) levels normalized to protein content and ratio of reduced NADPH versus oxidized NADP⁺ (H). Data indicate means ± SEMs (n = 6).

(I) Total number of colon tumors in mice fed with BHA and AOM/DSS treated. Data indicate means ± SEMs (Ucp2^+/+ n = 6, Ucp2^−/− n = 7).

(J) Size of individual colon tumors in mice fed with BHA and AOM/DSS treated, presented as means ± SEMs (Ucp2^+/+ n = 6, Ucp2^−/− n = 7).