Figure 5.

UCP2 Invalidation Increases HK2 Protein Expression and Decreases LDHA and G6PDH Activities in Colon Tumors

(A) Mitochondrial respiration rate was determined in the presence of 10 mM pyruvate, 5 mM malate, and 1.4 mM ADP. The leak was measured after adding 1 μg/mL oligomycin (Ucp2^+/+ T n = 10, Ucp2^−/− T n = 9).

(B) CS enzyme activity normalized to protein content. Data represent means ± SEMs (Ucp2^+/+ T n = 11, Ucp2^−/− T n = 7).

(C) Scheme showing the ¹⁴CO₂-producing reactions from [U-¹⁴C]-glucose (red) and [2-¹⁴C]-pyruvate (blue).

(D) [U-¹⁴C]-glucose (Ucp2^+/+ n = 11, Ucp2^−/− n = 12) and [2-¹⁴C]-pyruvate (Ucp2^+/+ n = 7, Ucp2^−/− T n = 9) oxidation into ¹⁴CO₂ of Ucp2^+/+and Ucp2^−/− colon tumors (T). Data indicate means ± SEMs.

(E) Immunoblots and quantifications of HK2 and LDHA expression using whole-cell extracts from colon tumors. Data indicate means ± SEMs (n = 8–12).

(F) LDH enzyme activity. Data represent means ± SEMs (Ucp2^+/+ n = 5, Ucp2^−/− n = 6).

(G) G6PDH enzyme activity relative to Ucp2^+/+ T (Ucp2^+/+ n = 7, Ucp2^−/− n = 8).

See also Figures S3 and S4.