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. 2019 Jun 10;8(3):475–486. doi: 10.1016/j.jcmgh.2019.06.002

Figure 1.

Figure 1

Changes in subcellular distribution of Rac1 protein in the HuR-deficient intestinal epithelium in vivo. (A) Immunohistochemical staining of Rac1 in small intestinal mucosa in control littermate and IE-HuR-/- mice. Green, signals of Rac1; blue, nuclei stained by 4′,6-diamidino-2-phenylindole (DAPI). Red arrows point to Rac1 localization; red box define the area to be amplified. Scale bar: 50 μm. (B) Representative immunoblots in cytoplasmic (left), nuclear (middle), and total (right) Rac1 protein in small intestinal mucosa. Cytoplasmic, nuclear, and total proteins were isolated from the small intestinal mucosa and prepared for Western blot analysis. Equal loading in total proteins was monitored by actin; the quality and loading of the cytoplasmic and nuclear fractions were monitored and examined by β-tubulin and lamin B, respectively. Three experiments were performed that showed similar results. (C) Quantitative analysis derived from densitometric scans of immunoblots of cytoplasmic (a) and total Rac1 (b) as described in panel B. Values are the means ± SEM of 3 separate experiments. *P < .05 compared with littermates.