Figure 5.

NPM directly interacts with Rac1 in IEC-6 cells. (A) Immunostaining of NPM and Rac1. Cells were permeabilized and incubated with either anti-NPM or anti-Rac1 antibody and then with anti-IgG antibody conjugated with Alexa Fluor. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Green, signals of NPM; red, signals of Rac1. Scale bar: 5 μm. (B) NPM/Rac1 association as measured immunoprecipitation (IP) assay using anti-Rac1 antibody (Ab). (C) HuR silencing decreases the levels of NPM/Rac1 complex without an effect on total Rac1 abundance. Cells were transfected with siHuR or C-siRNA, and total cell lysates were harvested 48 hours after the transfection. Three experiments were performed that showed similar results. Con, control.