Figure 11.

IL-10 is required by GPBAR1 to modulate the response of NKT cells. Acute hepatitis was induced in C57BL6 male mice, IL-10^+/+ and IL-10^–/–, by intravenous injection of Con A (15 mg/kg) at 8 hours. Mice were randomized to receive Con A alone or in combination with BAR501 (30 mg/kg) daily from 3 days before induction of hepatitis to the time of the sacrifice. Total RNA extracted from liver was used to evaluate by quantitative real-time PCR the relative mRNA expression of (A) proinflammatory genes IL-1β, TNF-α, IL-6, and IFN-γ; (B) anti-inflammatory genes TGF-β and IL-10; (C) adhesion molecules CXCR6 and LFA-1, FasL, and OPN; and markers specific for different immune cells populations such as (D) Cd49b, FoxP3, Cd38, and c-Myc. The data are normalized to GAPDH mRNA. Each point represents a single mouse. *P < .05.