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. 2019 Jun 18;8(3):447–473. doi: 10.1016/j.jcmgh.2019.06.003

Figure 12.

Figure 12

Effects of BAR501 on NKT cells in IL-10+/+and IL-10–/–mice. Acute hepatitis was induced in C57BL6 male mice, IL-10+/+ and IL-10–/–, by intravenous injection of Con A (15 mg/kg) at 8 hours. Mice were randomized to receive Con A alone or in combination with BAR501 (30 mg/kg) daily from 3 days before induction of hepatitis to the time of the sacrifice. type I and type II NKT cells were purified from the liver of mice with Con A–induced hepatitis, treated or untreated with BAR501. (A) Number of type I (left panel) and type II (middle panel) NKT cells and ratio between the number of type I and type II NKT cells (right panel). Real-time PCR analysis of (B) IFN-γ, IL-10, TNF-α, LFA-1, and OPN expression in type I NKT cells. Real-time PCR analysis of (C) IFN-γ, IL-10, and LFA-1 expression in type II NKT cells. Enzyme-linked immunosorbent assay test to evaluate IFN-γ and IL-10 production by NKT cells, were performed on culture supernatants. Data shown are (D) IFN-γ production by type I and type II NKT cells; (E) IL-10 production by type I and type II NKT cells. Results are the mean ± SEM of 6 points per group. *P < .05.