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. 2019 Aug 12;24(16):2916. doi: 10.3390/molecules24162916

Figure 6.

Figure 6

Bmal1 plays a critical role in RES-alleviated lipid metabolism misalignment initiated by FFA exposure in hepatic HepG2 cells. HepG2 cells were exposed to si-Ctrl or si-Bmal1 for 48 h, pretreated with or without RES (100 µM), and treated with FFA (100 µM) with 0.1% BSA for 24 h. (A) Representative western blot of Bmal1, p-AMPK, p-GSK3β, total AMPK, and total GSK3β after treatment with RES and FFA in HepG2 cells. (C) The expression levels of p-ACC, FAS, SREBP-1c, and PPARγ were detected in cells by western blot analysis. ACC and β-actin served as controls. (B) and (D) Densitometric analysis of the blots shown in (A) and (C), respectively. Data were presented as the mean ± SD, n ≥ 3. (∗) p < 0.05 and (∗∗) p < 0.01, versus control group; (#) p < 0.05 and (##) p < 0.01, versus FFA group; (△) p < 0.05 and (△△) p < 0.01, versus RES pretreatment with FFA group.