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. 2019 Aug 14;20(16):3954. doi: 10.3390/ijms20163954

Figure 5.

Figure 5

The combining ability of HI2, HW2, HV2, HF2, and PG-1 (1 μM, 2 μM, 4 μM, 8 μM, 16 μM, 32 μM, and 64 μM) with LPS. 50 μg/mL LPS from E. coli O111:B4 was incubated with 5 μg/mL BODIPY-TR cadaverine in Tris buffer (50 mM, pH 7.4) for 4 h at room temperature. Subsequently, the peptides were serially diluted in Tris buffer and incubated with equal volumes of the LPS-probe mixture for 1 h in a 96-well black plate. The fluorescence was measured (excitation λ = 580 nm, emission λ = 620 nm) on a spectrofluorophotometer.