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Outer membrane permeabilization assays of HV2 and PG-1. The uptake capacity of different concentrations of peptides to the NPN of E. coli ATCC 25922 was evaluated using a fluorescent dye (NPN) assay. The NPN uptake was monitored at an excitation wavelength of 350 nm and an emission wavelength of 420 nm. Polymyxin B was used as a positive control for its strong outer membrane permeability properties.
