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Summary of thrombin–TBA kinetic parameters in indicated buffers. Data represent the mean (±standard deviation, SD) of three independent experiments.
| Buffer | kON (107 M−1s−1) | kOFF (10−2 s−1) | KD (nM) |
|---|---|---|---|
| TE140-KCl | 38 ± 1.3 | 5.8 ± 0.1 | 0.15 ± 0.01 |
| TE140-NH4Cl | 17 ± 1.0 | 4.8 ± 0.1 | 0.28 ± 0.02 |
| TE140-NaCl | 3.3 ± 0.2 | 4.3 ± 0.1 | 1.31 ± 0.12 |
| TE140-LiCl | 0.03 ± 0.01 | 6.3 ± 0.4 | 245 ± 94 |
