Table 4.
Extraction efficiency, matrix effect and stability for UPLC-MS/MS assay of ophiopogonins in fermented broth of human gut microbiota.
| Analyte | Spiked (μM) | Extraction Efficiency (n = 5) | Matrix Effects (n = 5) | Autosampler Stability (n = 6) | |||
|---|---|---|---|---|---|---|---|
| Measured (%) | RSD (%) | Measured (%) | RSD (%) | Measured (%) | RSD (%) | ||
| Ophiopogonin D | 0.60 | 94.74 | 7.85 | 97.85 | 9.85 | 102.91 | 8.58 |
| 3.00 | 96.17 | 6.36 | 87.83 | 9.08 | 94.33 | 5.54 | |
| 30.0 | 104.61 | 13.05 | 89.92 | 5.81 | 99.13 | 12.64 | |
| Ophiopogonin D’ | 0.60 | 88.86 | 8.75 | 96.78 | 12.76 | 89.69 | 7.36 |
| 3.00 | 96.13 | 10.97 | 94.36 | 6.5 | 97.15 | 8.17 | |
| 30.0 | 98.66 | 10.34 | 96.89 | 6.95 | 101.59 | 11.39 | |
| Ruscogenin | 0.60 | 86.79 | 5.27 | 87.93 | 6.96 | 102.68 | 4.87 |
| 3.00 | 94.54 | 5.7 | 92.28 | 6.98 | 96.95 | 6.67 | |
| 30.0 | 99.32 | 3.46 | 97.06 | 6.11 | 94.70 | 6.58 | |
| Diosgenin | 0.60 | 90.17 | 9.08 | 92.29 | 7.89 | 102.60 | 6.52 |
| 3.00 | 86.50 | 5.72 | 94.90 | 7.39 | 97.04 | 3.65 | |
| 30.0 | 96.76 | 4.19 | 97.10 | 5.74 | 92.17 | 4.52 | |