Table 2.
Antibody specifications.
| Antibody | Protein Group | Catalogue Number | Dilution | HIER | Method | Secondary Antibody |
|---|---|---|---|---|---|---|
| Collagen type IV (α1/α2) | Collagens | Abcam, ab6586 | 1:4000 | low pH | IHC | HRP-coupled |
| Collagen type VI (α1) | Abcam, ab6588 | 1:1000 | low pH | IF | A-21246 | |
| Decorin | Proteoglycans | Atlas Antibodies, HPA003315 | 1:1000 | high pH | IHC | HRP-coupled |
| Biglycan | Atlas Antibodies, HPA003157 | 1:500 | high pH | IF | A-21246 | |
| Versican | Atlas Antibodies, HPA004726 | 1:500 | high pH | IF | A-21246 | |
| Periostin | Glycoprotein | Abcam, ab79946 | 1:1000 | low pH | IHC | HRP-coupled |
| Vimentin | Cytoskeleton | R&D, AF2105 | 1:200 | low pH | IF | A-21432 |
| Vinculin | Focal adhesions | Sigma-Aldrich, HPA063777 | 1:100 | low pH | IF | A-21246 |
HIER = heat induced epitope retrieval; HRP = horse-radish peroxidase; A = Alexa Fluor.