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. 2019 Aug 9;20(16):3894. doi: 10.3390/ijms20163894

Figure 3.

Figure 3

Heatmap representation of oxylipin production of n-6 and n-3 fatty acid-derived lipid mediators. (a) Astrocytes were treated with the high molecular weight hyaluronic acid (HMW, 450 µg/mL) and low molecular weight hyaluronic acid (LMW, 450 µg/mL) for 0.5 or 48 h, and LPS (100 ng/mL) or PIC (10µg/mL) for 4 h. Concentrations of oxylipins in supernatants were measured using UPLC-MS/MS. The heat map shows relative amounts of each lipid mediator compared to the control. The horizontal axis indicates the stimuli, while the vertical axis indicates the relative amount (ln) of each lipid mediator. (b) Astrocytes were pretreated for 0.5 or 48 h with HMW (HMW, 450 µg/mL) and (LMW, 450 µg/mL) HA and then stimulated with LPS (100 ng/mL) or PIC (10 µg/mL) for 4 h. The heat map shows relative amounts (ln) of each lipid mediator compared to the LPS and PIC-stimulated cells respectively. Values represent the mean from three independent experiments. Metabolites were divided into: Lipoxygenase (LOX), cyclooxygenase (COX) and cytochrome (CYP) pathways involved in their synthesis.