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. 2019 Aug 16;24(16):2971. doi: 10.3390/molecules24162971

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Respiratory chain complexes activity. Biochemical activities of succinate quinone DCPIP reductase (cII), NADH-cytochrome c oxidoreductase (cIII) and cytochrome c oxidase (cIV) were measured on a mitochondrial enriched fraction. mHtcum was added at increasing concentrations (from 50 to 150 µM) and compared to DMSO treated cells (CNT). Specific inhibitors for complex II (malonate), complex III (antimycin A) and complex IV (Na-azide) were used as control. Values were normalized to the control (CTN) and represented as the mean of three independent experiments ± S.D. (* p ≤ 0.05).