Table 4.
Studies dealing with lung injury (BALF: bronchoalveolar lavage fluid; BMDM: bone marrow-derived macrophages; PBEF: pre-B-cell colony-enhancing factor).
| Lung Injury | ||||
|---|---|---|---|---|
| Animals | ||||
| Study | Disease | Sample | miRNAs | Findings |
| Xu et al. (2014) [68] | Lung injury | Animals: healthy male C57BL/6 mice. Cells: Epithelial cells |
miR-17 | miR-17 antagomir increased the expression of FoxA1 in Acute Lung Injury mice (p < 0.05) |
| Yuan et al. (2015) [32] | Lung inflammation | Animals: male wild-type C57BL/6J mice. Cells: bone marrow-derived macrophages |
miR-155 | Increased expression of miR155 by mTREM-1 suppressed by antimiR-155 (p < 0.05) |
| Fu et al. (2018) [45] | Pulmonary inflammation | Animals: male BALB/c mice. Cells: murine macrophage RAW264.7 cells |
miR-92a | Antagomir-92a reduced pathological changes associated with lung inflammation, reduces lung wet/dry ratio (p < 0.01), and Evans blue dye extravasation (p < 0.01). Inhibition of miR-92a reduced the repression of TNF-α, IL-1β, IL-6 (p < 0.01) in lung tissues |
| Wu et al. (2018) [67] | Acute lung injury | MK2 deficient mice (C57BL/6) (B6.129P2-Mapkapk2tm1Dgen/J, and MK2flox/flox mice | Let-7e | Transfection of anti-let-7e into MK2-/- BMDM rescued LPS-induced expression of TNF-α, IL-6, and MIP-2 (p < 0.05) |
| Xie et al. (2018) [79] | Lung inflammation, lung injury | Animals: male C57BL/6 mice. Cells: RAW264.7 cells |
miR-34b-5p | miR-34b-5p antagomir in vivo inhibited miR-34b-5p up-regulation, reduced inflammatory cytokine release, decreased alveolar epithelial cell apoptosis, attenuated lung inflammation, improved survival by targeting PGRN during acute lung injury (p < 0.05) |
| Huang et al. (2019) [47] | Acute Lung Injury | Sixty healthy male-specific pathogen free C57BL/6 mice | miR-27b | Downregulation of miR-27b decreased the levels of IL-1β, IL-6, and TNF-α in BALF of Acute Lung Injury mice (p < 0.05) |
| Cell Lines | ||||
| Study | Disease | Sample | miRNAs | Findings |
| Adyshev et al. (2013) [80] | Lung injury | Human pulmonary artery endothelial cells | hsa-miR-374a, hsa-miR-374b, hsa-miR-520c-3p, hsa-miR-1290 | Antagomirs for each MYLK miRNA increased 3′UTR luciferase activity (1.2–2.3 FI) and rescued the decreased MLCK-3′UTR reporter activity produced by miRNA mimics (70%–110% increases for each miRNA; p < 0.05) |
| Adyshev et al. (2014) [81] | Lung inflammation | Human pulmonary artery endothelial cells | hsa-miR-374a, hsa-miR-568 | Antagomirs for each PBEF/NAMPT miRNA increased the endogenous PBEF/NAMPTmRNA and protein levels and 3′-UTR luciferase activity compared with controls (p < 0.05) |
| Xu et al. (2014) [68] | Lung injury | Animals: healthy male C57BL/6 mice. Cells: epithelial cells |
miR-17 | miR-17 antagomir increased the expression of FoxA1 in Acute Lung Injury mice (p < 0.05) |
| Yuan et al. (2015) [32] | Lung inflammation | Animals: Male wild-type C57BL/6J mice. Cells: bone marrow-derived macrophages |
miR-155 | Increased expression of miR155 by mTREM-1 suppressed by antagomir against miR-155 (p < 0.05) |
| Fu et al. (2018) [45] | Pulmonary inflammation | Animals: male BALB/c mice. Cells: murine macrophage RAW264.7 cells |
miR-92a | Antagomir-92a reduced pathological changes associated with lung inflammation, reduces lung wet/dry ratio (p < 0.01), and Evans blue dye extravasation (p < 0.01). Inhibition of miR-92a ameliorated the inflammatory response by reducing the repression of TNF-α, IL-1β, IL-6 (p < 0.01) in lung tissues |
| Xie et al. (2018) [79] | Lung inflammation, lung injury | Animals: male C57BL/6 mice. Cells: RAW264.7 cells |
miR-34b-5p | miR-34b-5p antagomir in vivo inhibited miR-34b-5p up-regulation, reduced inflammatory cytokine release, decreased alveolar epithelial cell apoptosis, attenuated lung inflammation, improved survival by targeting PGRN during acute lung injury (p < 0.05) |