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1H-NMR of α-Syn treated with Cu(II), EGCG, or their mixture. (A) The aromatic region of α-Syn within 6.55 and 8.65 ppm; (B) high field region of α-Syn and EGCG within 0.55 and 3.15 ppm. Samples are 50 μM α-Syn incubated for 0 h (1) and 29 h (2) alone, in the presence of 40 μM Cu(II) (3), 20 μM EGCG (4), the mixture of 10 (5), 20 (6), 40 (7) μM Cu(II) with 20 μM EGCG, respectively, for 58 h in PBS buffer (20 mM, pH 7.4).
