Figure 2.

Analysis of the sensors’ ability to be regenerated. All four sensors (sensor-I to sensor-IV) at 30 nM concentration in 1 × TAE-Mg buffer were tested for bulk fluorescence resonance energy transfer (FRET) efficiency change upon addition of 60 nM target and 20 min incubation. For probe regeneration, two-fold excess of either the target or the probe strands were added in each subsequent steps. While sensor-I and sensor-II showed no to poor regeneration, sensors III and IV were fully regenerated (n = 3, errors bars are too small to be visible). All of the experiments were performed at room temperature (23 °C).