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. 2019 Aug 15;20(16):3976. doi: 10.3390/ijms20163976

Figure 2.

Figure 2

Analysis of transient expression of green fluorescent protein (GFP) in cassava leaves. (A) At 2 days postinfiltration (dpi), Agrobacterium with no plasmid was used as the mock, and Agrobacterium containing 35S::GFP was used as the sample. Bar = 25 μm. (B) The relative transcript level of GFP is shown by reverse-transcription PCR, and elongation factor 1 (EF1) was used as a reference gene. (C) GFP was detected by Western blot assay, 10 μg total protein extracted from infiltrated leaves were loaded onto SDS-PAGE gel, and Coomassie staining shows the equal protein content.