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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Exp Hematol. 2019 Jun 28;75:53–63.e11. doi: 10.1016/j.exphem.2019.06.473

Figure 4. Treatment of PBSC CD34+ cells with TSA modulates expression of stem cell related genes.

Figure 4.

(A) Effects of TSA treatment on the relative transcript level of genes (GATA1, GATA2, NOTCH1, BMI1, HOXB4, SALL4, PU.1, PU.1, PTEN, BCL2, c-MYC, and MPO) were measured by real-time quantitative PCR. Total RNA was extracted from cells obtained after 3 days of culture in the presence of cytokines with TSA or DMSO. GAPDH was used as internal calibrator (control gene). Measurements were obtained in duplicate using at least 2 independent samples. (B) TSA treated CD34+CD90+ cells and CD34+CD90− cell were sorted and the expression levels of GATA1, GATA2, HOXB4, and SALL4 were analyzed. Measurements were obtained in duplicate using at least 2 independent samples. Error bars indicate SE; * indicates p<0.05.