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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Exp Hematol. 2019 Jun 28;75:53–63.e11. doi: 10.1016/j.exphem.2019.06.473

Figure 5. SALL4 silencing decreased TSA-mediated expansion of CD34+CD90+ cells.

Figure 5.

(A) The strategy of knockdown of SALL4 and GATA2. (B) Transduction efficacy was evaluated by using lentiviral vectors pLL3.7 and pLKO.3G expressing GFP. (C) SALL4 mRNA expression after shRNA targeting in PBSC CD34+ cells (D) GATA2 mRNA expression after shRNA targeting. (E) The percentage of TSA-mediated CD34+CD90+ cells after SALL4 silencing. (F) The percentage of TSA-mediated CD34+CD90+ cells after GATA2 silencing. (N=3, Error bars indicate SD)