Table 1.
Schematic diagram of the patient’s timeline (adjusted from SPIRIT guidelines for trial protocols)
| Timepoint | Study period | ||||
|---|---|---|---|---|---|
| Enrolment | Biopsy | Close-out | |||
| Transplantation | For cause | M3 | M12 | M24 | |
| Enrolment: | |||||
| Eligibility screen | X | ||||
| Informed consent | X | ||||
| Interventions: | |||||
| PBMC collection | X | X | X | X | |
| Kidney Biopsy | X | X | X | ||
| Assessments: | |||||
| TCR repertoire sequencing of unstimulated PBMCs | X | X | X | X | |
| TCR sequencing of Alloreactive T cells after MLR | X | ||||
| Phenotypic analysis of peripheral T cell populations | X | X | X | X | |
M3 Month 3, M12 Month 12, MLR Mixed lymphocyte reaction, TCR T cell receptor, PBMC Peripheral blood mononuclear cells