Table 3.

Molecular analysis of H5N6 virus isolates in Fujian Province, China

Viral Protein	Mutations#	Virus1	Virus2	Virus3	Virus4	Virus5	Virus6	Virus7	Virus8	Function
HA	Q226L*	Q	Q	Q	Q	Q	Q	Q	Q	Increased α2,6-SA recognition [22]
	G228S*	G	G	G	G	G	G	G	G	Increased α2,6-SA recognition [22]
	Cleavage site	RERRR KR↓GL	RERRR KR↓GL	RERRR KR↓GL	RERRR KR↓GL	RERRR KR↓GL	RERRR KR↓GL	REKRR KR↓GL	RERRR KR↓GL	Enhanced pathogenic effects in poultry [23]
NA	69-73deletion	No	No	No	No	No	No	No	No	Increased virulence in mice [24]
	R294K/N294S	R	R	R	R	R	R	R	N	Oseltamivir and zanamivir resistance [25]
PB2	L89V	V	V	V	V	V	V	V	V	Enhanced pathogenicity in mice [26]
	G309D	D	D	D	D	D	D	D	D	Increased virulence and replication in mice [27]
	T339K	K	K	K	K	K	K	K	K	Increased virulence and replication in mice [27]
	R477G	G	G	G	G	G	G	G	G	Increased virulence and replication in mice [27]
	I495V	V	V	V	V	V	V	V	V	Increased virulence and replication in mice [27]
	E627K	E	E	E	E	E	E	E	E	Mammalian host adaptation [28]
	D701N	D	D	D	D	D	D	D	D	Enhanced transmission in guinea pigs [29]
PB1	H99Y	H	H	H	H	H	H	H	H	Enhanced H5 virus transmissible among ferrets [26]
	I368V	I	I	I	V	I	I	I	I	Enhanced H5 virus transmissible among ferrets [26]
NS1	P42S	S	S	S	S	S	S	S	S	Increased virulence in mice [30]
	D92E	E	E	E	D	E	E	D	D	Increased virulence in mice and pigs [27]
	218-230deletion	Yes	Yes	Yes	Yes	Yes	Yes	No	No	Lack of PDZ domain binding motif:decreased virulence in mice [31]
M1	N30D	D	D	D	D	D	D	D	D	Increased virulence in mice [32]
	T215A	A	A	A	A	A	A	A	A	Increased virulence in mice [32]
M2	S31N	S	S	S	N	S	S	S	S	Amantadine resistance [33]