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. 2019 Aug 1;151(9):1116–1134. doi: 10.1085/jgp.201912327

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© 2019 Sato et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 3. — Time course of the formation of Ca_V1.2 channel clusters in tsA-201 cells. (A) Representative superresolution GSD binary masks of immunolabeled Ca_V1.2 channels expressed in tsA-201 cells at 0, 12, 24, 36, and 48 h after transfection. (B) Frequency distribution of Ca_V1.2 channels cluster areas at each time point. (C) Relative frequency distribution of Ca_V1.2 channels cluster areas expressed as percentages. Dashed color lines in B and C represent the best single exponential decay function fit for each dataset. (D) Change of mean Ca_V1.2 channel cluster area at different time points after transfection. Data are presented as mean ± SEM for individual cells at each time point. Mean cluster area data were fit with a one-phase exponential equation (solid black line). The tau value of the fit is 8.37 h. (E) Change of Ca_V1.2 channel cluster density at different time points after transfection. Data are presented as mean ± SEM for individual cells at each time point. Mean cluster density data were fit with a sigmoidal function (solid black line). The logIC₅₀ fit is 11.42 h. (B–E) All data were compiled as follows: 4 h: n = 1,497 clusters from 4 cells; 6 h: n = 2,100 clusters from 4 cells; 12 h: n = 87,209 clusters from 16 cells; 24 h: n = 114,805 clusters from 11 cells; 36 h: n = 66,769 clusters from 5 cells; 48 h: n = 118,281 clusters from 13 cells.