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. 2019 Aug 1;151(9):1116–1134. doi: 10.1085/jgp.201912327

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© 2019 Sato et al.

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Figure 5. — Dwell times for Ca_V1.2 and TRPV4 channels in tsA-201 cells are fast. (A) Representative image of a tsA-201 cell transfected with Ca_V1.2-PA-GFP immediately after stimulation (i), with corresponding labeled ROI slices (ii), and fluorescent traces of the ROI slices (iii). F, fluorescence. (B) Mean time course of Ca_V1.2-PA-GFP normalized fluorescence (dark green dashed line) and SEM at each time (light green shaded area) of all normalized ROIs. Time course was fit with an exponential function (black line). 26 ROIs from nine cells were averaged. (C) Representative image of a tsA-201 cell transfected with TRPV4-PA-GFP immediately after stimulation (i), with corresponding labeled ROI slices (ii), and fluorescent traces of the ROI slices (iii). (D) Mean time course of TRVP4-PA-GFP normalized fluorescence (dark green dashed line) and SEM at each time (light green shaded area) of all normalized ROIs. Time course was fit with an exponential function (black line). 17 ROIs from 6 cells were averaged.