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. 2019 Aug 13;151(9):1135–1145. doi: 10.1085/jgp.201912421

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© 2019 Pandhare et al.

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Figure 3. — Weight determination of ICD chimeras by SDS-PAGE and SEC. (A) The weight of the ICD chimeras in monomeric state was determined by resolving SEC-purified protein on SDS-gel electrophoresis. Molecular weight standards are shown on top, and ICD (53 kD), ΔMA (49 kD), MA (45 kD), Δ44 (48 kD), and QDA (52 kD) are shown below. (B) The complete SDS-gel electrophoresis image from which the analysis in section A is drawn. A standard protein ladder is shown next to each protein for increased accuracy of the analysis. Lane compositions are as follows: lane 2, ICD; lane 4, ΔMA; lane 6, MA; lane 8, Δ44; and lane 10, QDA. (C) SEC yielded a weight of 267 kD for ICD, 261 kD for Δ44, 180 kD for ΔMA, 141 kD for QDA, and 73 kD for MA. Chromatogram of standard proteins (T, thyroglobulin; F, ferritin; A, aldolase; C, conalbumin; O, ovalbumin) in gray, and chimeras in their respective colors are shown above. A280, UV absorbance at 280 nm.