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. 2019 Jul 3;216(9):2113–2127. doi: 10.1084/jem.20181454

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© 2019 Prager et al.

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Figure 5. — Changes in granule content and surface CD95L on NK cells during serial killing. Primary human NK cells were incubated at an E/T ratio of 2 with HeLa-CD48 cells in the presence of an anti-CD107a antibody. After the indicated times, NK cells were harvested and stained for CD45, CD95L, and TRAIL followed by intracellular staining for perforin and GrzB. Unstained NK cells (control) and stained NK cells incubated for 4 h without target cells (NK cells) were used as controls. (A and C) Histograms for the staining of CD95L, TRAIL, perforin, and GrzB on CD107a⁺ (A) and CD107a⁻ (C) NK cells (gated on CD45⁺). (B and D) Summary of three independent experiments showing the mean fluorescence staining intensity (MFI) for CD95L or the relative fluorescence staining intensity (RFI, normalized to the NK cell only control) for TRAIL, perforin, and GrzB at the different time points for CD107a⁺ (B) and CD107a⁻ (D) NK cells. Values shown are the mean ± SD; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (one-way ANOVA).

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