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. 2019 Jul 3;216(9):2113–2127. doi: 10.1084/jem.20181454

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© 2019 Prager et al.

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Figure 6. — Perforin is essential for NK cell serial killing. (A) Intracellular FACS staining of perforin in human NK cells before (control) and after CRISPR/Cas9-mediated KO. (B) Example for intracellular perforin staining in KO and WT clones. (C) Perforin WT (C) and KO (D) NK cell clones (NK cells from two independent clones each) were evaluated against HeLa-CD48 cells stably expressing NES-ELQTD-GFP-T2A-NES-VGPD-mCherry. NK cells were tracked over 15–17 h, and each of their killing events was characterized in terms of GrzB and Casp8 activity as well as the kinetics of reporter cleavage and cell death.

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