Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 21;216(9):2128–2149. doi: 10.1084/jem.20190249

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Clarke et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 6. — Surface TIM-3⁺IL-7R⁻ status uniquely characterizes a set of tumor T_RM cells. (A) tSNE visualization of flow cytometry data from 3,000 randomly selected live and singlet-gated CD14⁻CD19⁻CD20⁻CD56⁻CD4⁻CD45⁺CD3⁺CD8⁺ cells isolated from eight paired tumor and lung samples; each cell is represented by a dot colored as T_RM or non-T_RM (left), tumor or lung (second and third from left), and according to z-score expression value of the protein indicated above the plot (remaining panels). (B) Left: Contour plots show the expression of TIM-3 versus IL-7R in the cell type and tissue indicated above the plot; percentage of tumor CTLs (gated as above) in the indicated populations that express TIM-3 is shown. Right: Quantification of TIM-3⁺ in cells isolated from each tissue location; each symbol represents an individual sample. The small line indicates SEM, and bars represent the mean and are colored as indicated (*, P ≤ 0.05; n = 8, 3). (C) Geometric mean fluorescent intensity (GMFI) of CD39, PD-1, and 4-1BB for each lung tumor-T_RM subset; bars represent the mean, t-lines represent SEM, and each symbol represents data from individual samples (**, P ≤ 0.01; n = 8; Wilcoxon rank-sum test); representative histograms are shown at left. (D) Coexpression analysis of flow cytometry data (C), as per Spearman correlation. (E) Contour plot highlighting the expression of PD-1 versus TIM-3 in CD14⁻CD19⁻CD20⁻CD56⁻CD4⁻CD45⁺CD3⁺CD8⁺CD103⁺ cells in a representative donor. (F) Analysis of three HNSCC samples, as per B. (G) Left: Representative multiplexed immunohistochemistry of CD8A, CD103, and TIM-3 in tumor specimens from a patient with TIL^low/T_RM^low tumor status; the representative false color image (middle) and the overlaid image (bottom) are shown. Right: As the left plots for a patient with TIL^high/T_RM^high tumor status (scale bar, 50 µm). (H) Left: Quantification of the number of CD8A⁺CD103⁺TIM-3⁺ cells per region in biopsies defined as having a TIL^high/T_RM^high status versus TIL^low/T_RM^low status. Right: Percentage of CD8A⁺CD103⁺ CTLs expressing TIM-3 in each clinical subtype. Bars represent the mean, t-lines represent SEM, and symbols represent individual data points (*, P ≤ 0.05; ****, P ≤ 0.0001; n = 21; Mann–Whitney U test).