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. 2019 Jul 16;216(9):2170–2183. doi: 10.1084/jem.20181953

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© 2019 Moser et al.

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Figure 2. — Itch acts within B cells to negatively regulate GC B cell numbers. (A) Experimental design. (B) Percent of WT (B6.SJL [CD45.1]) and Itch KO (CD45.2) cells within immune populations was enumerated by flow cytometry (n = 6, compiled from two independent experiments, two-way ANOVA with Sidak post-test). (C) Percent of GC B cells of each genotype was divided by percent of FO B cells from the same genotype (n = 6 compiled from two independent experiments, paired t test). (D) NP⁺ GC B cells were analyzed on day 14 after immunization. Percent of NP⁺ GC B cells from each genotype was divided by percent FO B cells from the same genotype (n = 4, compiled from two independent experiments, paired t test). (E) Equal numbers of flow cytometry–sorted CD45.1⁺ and CD45.2⁺ cells were plated on an NP-BSA–coated ELISPOT plate, and IgG1-secreting PCs were enumerated (n = 5, compiled from two independent experiments, paired t test). For A–E, two sets of BM donors were used to generate the chimeras. *, P < 0.05; **, P < 0.01; ****, P < 0.0001.