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. 2019 Jul 16;216(9):2170–2183. doi: 10.1084/jem.20181953

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© 2019 Moser et al.

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Figure 3. — Proteomic profile of Itch KO B cells. (A) Itch-deficient (Itch/IL4 DKO) and control (IL4 KO) FO B cells were stimulated for 24 h with 0.1 μM CpG. Cells were lysed, fractionated, trypsin-digested, and subjected to liquid chromatography and tandem mass spectrometry analysis followed by protein assignation and quantification by MaxQuant. Normalized intensity-based absolute quantification values (for protein abundance) were compared between genotypes to generate fold change. Red dots indicate 163 proteins that were statistically significantly different (n = 3, t test corrected for repeated measures, P < 0.05, IL4 KO mice were on a B6.SJL background [CD45.1]). (B) Top Molecular Signatures Database hallmarks indicated by the 163 proteins that were different between Itch KO and control cells. FDR, false discovery rate. (C) Gene names for the proteins within the top hallmark (genes up-regulated through activation of mTORC1). (D) Gene names for the proteins within the second-ranked hallmark (cell cycle–related targets of E2F transcription factors).