Figure 6.

Endogenous Elys associates with Drosophila PBAP and regulates chromatin compaction. (A) Right: Coimmunoprecipitation experiments in S2 cell lysates, in which immunoprecipitates of the components of the PBAP complex were Western blotted for Elys, Sec13, and Nup98. Left: Coimmunoprecipitation experiments, in which immunoprecipitates of Elys or Sec13 were Western blotted for components of the PBAP complex. 10% of lysate relative to immunoprecipitate was loaded for inputs, 40% per sample. The experiment was done three times, and representative blots are shown. (B) Representative gel image of genomic DNA subjected to MNase digestion for indicated lengths of time from S2 cells treated with dsWhite (control), dsSec13, or dsElys RNAi (for 6 d). Black boxes indicate the mononucleosome band, used in quantification of digestion (in C), relative to the undigested genomic band at the top. (C) Quantification of Mnase digestion of chromatin harvested from S2 cells treated with control, Elys, or Sec13 dsRNA, displayed as a plot of relative amounts of the detected mononucleosome band and the undigested genomic band, at the indicated times of digestion. The mean and standard error bars are calculated from four independent biological replicates (two replicates from two independent experiments). *, P < 0.05.