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. 2019 Aug 6;218(9):3098–3116. doi: 10.1083/jcb.201903124

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© 2019 Jayadev et al.

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Figure 2. — The pharyngeal BM is collagen rich, while the gonadal BM is laminin rich. Fluorescence images of collagen::mCh and laminin::mNG in the pharynx and gonad of 72-h adult animals. As laminin::mNG localizes strongly to the nerve ring BM that surrounds and contacts the pharyngeal BM at several regions (bracket), we confined our measurements of laminin signal to the dotted box where clear pharyngeal BM laminin is visible in this and all relevant figures, except Fig. 6 C, where laminin signal in the solid box was also measured. Quantification of collagen::mCh and laminin::mNG levels in the pharyngeal (collagen::mCh, n = 11; laminin::mNG, n = 15) and gonadal BMs (collagen::mCh, n = 20; laminin::mNG, n = 17) are shown in boxplots at the bottom. ***, P < 0.0001, unpaired two-tailed Student’s t test. Box edges in boxplots depict the 25th and 75th percentiles, the line in the box indicates the median value, and whiskers mark the minimum and maximum values. Scale bar, 10 µm. A.U., arbitrary units.