Skip to main content
. 2019 Aug 6;218(9):3098–3116. doi: 10.1083/jcb.201903124

Figure 4.

Figure 4.

The INA-1/PAT-3 integrin heterodimer is required for laminin-dependent collagen recruitment to the gonadal BM. (A) Fluorescence images of the integrin β subunit PAT-3::mNG and the integrin α subunits INA-1::mNG and PAT-2::2xmNG in the gonad. Magenta arrowheads indicate enrichment of fluorescence signal at the cell–BM interface. (B) Fluorescence images of gonadal BM collagen::mCh (left) and laminin::mNG (right) in control versus pat-3, ina-1, and pat-2 RNAi-treated 72-h adult animals. Regions of the BM in the dotted boxes are magnified in insets. Quantification of collagen::mCh (control, n = 38; pat-3 RNAi, n = 34; ina-1 RNAi, n = 34; pat-2 RNAi, n = 31) and laminin::mNG (control, n = 11; pat-3 RNAi, n = 17; ina-1 RNAi, n = 15; pat-2 RNAi, n = 17) fluorescence intensity for all treatments is shown at the bottom. ***, P < 0.0001, one-way ANOVA followed by post hoc Dunnett’s test. Box edges in boxplots depict the 25th and 75th percentiles, the line in the box indicates the median value, and whiskers mark the minimum and maximum values. Scale bars, 10 µm. A.U., arbitrary units.